Orthogonal optogenetic triple-gene control in Mammalian cells.

  title={Orthogonal optogenetic triple-gene control in Mammalian cells.},
  author={Konrad M{\"u}ller and R. Engesser and Jens Timmer and Matias D. Zurbriggen and Wilfried Weber},
  journal={ACS synthetic biology},
  volume={3 11},
Optogenetic gene switches allow gene expression control at an unprecedented spatiotemporal resolution. Recently, light-responsive transgene expression systems that are activated by UV-B, blue, or red light have been developed. These systems perform well on their own, but their integration into genetic networks has been hampered by the overlapping absorbance spectra of the photoreceptors. We identified a lack of orthogonality between UV-B and blue light-controlled gene expression as the… CONTINUE READING