Orthogonal‐plane fluorescence optical sectioning: Three‐dimensional imaging of macroscopic biological specimens

@article{Voie1993OrthogonalplaneFO,
  title={Orthogonal‐plane fluorescence optical sectioning: Three‐dimensional imaging of macroscopic biological specimens},
  author={Arne H. Voie and David H. Burns and Francis A. Spelman},
  journal={Journal of Microscopy},
  year={1993},
  volume={170}
}
An imaging technique called orthogonal‐plane fluorescence optical sectioning (OPFOS) was developed to image the internal architecture of the cochlea. Expressions for the three‐dimensional point spread function and the axial and lateral resolution are derived. Methodologies for tissue preparation and for construction, alignment, calibration and characterization of an OPFOS apparatus are presented. The instrument described produced focused, high‐resolution images of optical sections of an intact… 

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