Origins and unconventional behavior of neutrophils in developing zebrafish.

@article{LeGuyader2008OriginsAU,
  title={Origins and unconventional behavior of neutrophils in developing zebrafish.},
  author={Doroth{\'e}e Le Guyader and Michael J. Redd and Emma Colucci-Guyon and Emi Murayama and Karima Kissa and Val{\'e}rie Briolat and Elodie Mordelet and Agust{\'i}n G Zapata and Hiroto Shinomiya and Philippe Herbomel},
  journal={Blood},
  year={2008},
  volume={111 1},
  pages={
          132-41
        }
}
The first leukocytes that arise in the development of vertebrate embryos are the primitive macrophages, which differentiate in the yolk sac and then quickly invade embryonic tissues. These macrophages have been considered to constitute a separate lineage, giving rise to no other cell type. Using an in vivo photoactivatable cell tracer in the transparent zebrafish (Danio rerio) embryo, we demonstrated that this lineage also gave rise to an equal or higher number of neutrophilic granulocytes. We… 

Figures from this paper

spib is required for primitive myeloid development in Xenopus
TLDR
It is concluded that spib sits at the top of the known genetic hierarchy that leads to the specification of primitive myeloid cells in amphibians.
Labeling primitive myeloid progenitor cells in Xenopus.
TLDR
This work describes two methods for labeling primitive myeloid progenitor cells during early Xenopus development and describes their roles in the development and behavior of PMCs.
Trim33 is essential for macrophage and neutrophil mobilization to developmental or inflammatory cues
TLDR
It is discovered that in Trim33-deficient zebrafish embryos, primitive macrophages are unable to colonize the central nervous system to become microglia, and the transcriptional regulator Trim 33 is revealed as being essential for the navigation of macrophage and neutrophils towards developmental or inflammatory cues within vertebrate tissues.
Irf8 regulates macrophage versus neutrophil fate during zebrafish primitive myelopoiesis.
TLDR
It is shown that during zebrafish embryogenesis interferon regulatory factor-8 (irf8) is expressed specifically in macrophages but not neutrophils, and that the skewed myeloid lineage development in Irf8 knockdown embryos results from a cell-fate switching.
Cebpα is essential for the embryonic myeloid progenitor and neutrophil maintenance in zebrafish.
Live cell imaging of zebrafish leukocytes.
TLDR
Live imaging during the different stages of inflammation using this expanding library of reporter lines, coupled with the ability to model aspects of human disease in the zebrafish system, have the potential to provide significant insights into inflammation and diseases associated with its dysregulation.
Molecular Insights Into Neutrophil Biology From the Zebrafish Perspective: Lessons From CD18 Deficiency
TLDR
CD18 knock-out zebrafish larvae displayed the key symptoms of patients suffering from leukocyte adhesion deficiency (LAD) type I due to defects in ITGB2, the gene for CD18, and introduce a new model organism for studying LAD type I.
...
...

References

SHOWING 1-10 OF 35 REFERENCES
Ontogeny and behaviour of early macrophages in the zebrafish embryo.
TLDR
The results support the notion that in vertebrate embryos, macrophages endowed with proliferative capacity arise early from the hemopoietic lineage through a non-classical, rapid differentiation pathway, which bypasses the monocytic series that is well-documented in adult hemopOietic organs.
Zebrafish early macrophages colonize cephalic mesenchyme and developing brain, retina, and epidermis through a M-CSF receptor-dependent invasive process.
TLDR
It is shown that a specific lineage of early macrophages that differentiate in the yolk sac before the onset of blood circulation spread in the whole cephalic mesenchyme, and from there invade epithelial tissues: epidermis, retina, and brain--especially the optic tectum.
Early hematopoiesis and developing lymphoid organs in the zebrafish
TLDR
The initial appearance of various blood lineages and the nature of accumulating hematopoietic tissue in the thymus and kidney, the main lymphoid organs of adult teleosts, in zebrafish is described.
Myelopoiesis in the zebrafish, Danio rerio.
TLDR
The morphologic and molecular genetic evidence presented here supports the zebrafish as an informative model system for the study of normal and aberrant human myelopoiesis and shows high level of conservation of the spatio-temporal expression patterns of these genes between zebra fish and mammals.
Live imaging of emerging hematopoietic stem cells and early thymus colonization.
TLDR
In CD41-green fluorescent protein (GFP) transgenic zebrafish embryos, the transgene is expressed by emerging HSCs in the aorta-gonad-mesonephros (AGM) area, allowing for the first time to image their behavior and trace them in real time.
Morphologic and functional characterization of granulocytes and macrophages in embryonic and adult zebrafish.
TLDR
The early onset of zebrafish granulopoiesis is demonstrated, it is proved that granulocytes circulate by 48 hpf, and the functional activity of embryonic granulocyte and macrophages is demonstrated.
Three pathways to mature macrophages in the early mouse yolk sac.
TLDR
The combined data of phenotypic, gene-expression, and in situ analyses allowed us to conclude that the previously named "primitive Mphi" corresponded to a mixture of the first transient wave and committed Mphi precursors.
The cloche and spadetail genes differentially affect hematopoiesis and vasculogenesis.
TLDR
The studies demonstrate that the molecular mechanisms that regulate hematopoietic and vasculogenic cells in normal zebrafish have been conserved throughout vertebrate evolution and the clo and spt genes are key regulators of these programs.
...
...