Origin of the ectoplacental cone and secondary giant cells in mouse blastocysts reconstituted from isolated trophoblast and inner cell mass.

@article{Gardner1973OriginOT,
  title={Origin of the ectoplacental cone and secondary giant cells in mouse blastocysts reconstituted from isolated trophoblast and inner cell mass.},
  author={Richard L. Gardner and Virginia E Papaioannou and Sheila C. Barton},
  journal={Journal of embryology and experimental morphology},
  year={1973},
  volume={30 3},
  pages={561-72}
}
1. Inner cell mass (ICM) and trophoblast tissue were isolated from 3^-day post-coitum mouse blastocysts that were homozygous for different electrophoretic variants of the enzyme glucose phosphate isomerase (GPI). Blastocysts were reconstituted from these tissues, transferred to pseudo-pregnant recipients and allowed to develop to the early somite stage. 2. The embryo plus membranes and trophoblast were dissected and typed separately for GPI. 3. Contamination of trophoblast with maternal… CONTINUE READING

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