Origin of D-amino acids detected in the acid hydrolysates of purified Escherichia coli β-galactosidase.


In previous report, we detected D-amino acids in the acid hydrolysates of purified recombinant β-galactosidase. Here, we employed a deuterium-hydrogen exchange method to discriminate innate D-amino acids from those generated during hydrolytic incubation. After hydrolysis of β-galactosidase in DCl/D2O, amino acids were derivatized with NBD-F and separated on… (More)
DOI: 10.1016/j.jpba.2015.04.022


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