Oriented immobilization of farnesylated proteins by the thiol-ene reaction.

  title={Oriented immobilization of farnesylated proteins by the thiol-ene reaction.},
  author={Dirk Weinrich and Po-Chiao Lin and Pascal Jonkheijm and Uyen Tran Tu Nguyen and Hendrik V Schr{\"o}der and Christof M. Niemeyer and Kirill Alexandrov and Roger S. Goody and Herbert Waldmann},
  journal={Angewandte Chemie},
  volume={49 7},
Anchoring the protein: Proteins were immobilized rapidly under mild conditions by thiol-ene photocoupling between S-farnesyl groups attached to a genetically encodable “CAAX-box” tetrapeptide sequence (A is aliphatic) at the C terminus of the protein and surface-exposed thiols (see scheme). This method enables the oriented covalent immobilization of proteins directly from expression lysates without additional purification or derivatization steps. 

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