Oriented immobilization of epidermal growth factor onto culture substrates for the selective expansion of neural stem cells.

@article{NakajiHirabayashi2007OrientedIO,
  title={Oriented immobilization of epidermal growth factor onto culture substrates for the selective expansion of neural stem cells.},
  author={Tadashi Nakaji-Hirabayashi and Koichi Kato and Yusuke Arima and Hiroji Iwata},
  journal={Biomaterials},
  year={2007},
  volume={28 24},
  pages={
          3517-29
        }
}
To develop a culture substrate that allows efficient expansion of neural stem cells (NSCs), epidermal growth factor (EGF) was immobilized onto the Ni(II)-chelated surface of a glass-based substrate through coordination of Ni(II) to the histidine tag that was fused to the C-terminal of EGF using recombinant technology. For the preparation of the nickel-chelated surface, a thin gold layer was deposited to the glass surface, and then the self-assembled monolayer of alkanethiol terminated with… CONTINUE READING
BETA

Similar Papers

Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 40 CITATIONS

Design of cell culture substrates for large-scale preparation of neural cells

VIEW 5 EXCERPTS
CITES METHODS & BACKGROUND
HIGHLY INFLUENCED