Oriented binding of a lipid-anchored cell adhesion protein onto a biosensor surface using hydrophobic immobilization and photoactive crosslinking.

@article{Stein1996OrientedBO,
  title={Oriented binding of a lipid-anchored cell adhesion protein onto a biosensor surface using hydrophobic immobilization and photoactive crosslinking.},
  author={Torsten Stein and G{\"u}nther Gerisch},
  journal={Analytical biochemistry},
  year={1996},
  volume={237 2},
  pages={252-9}
}
The carboxymethyl-dextran surface of a biosensor instrument was modified to couple, in an active state, the lipid-anchored contact site A (csA) glycoprotein, a homophilic adhesion molecule of aggregating cells of Dictyostelium discoideum. The carboxy groups were modified by heptyl residues for hydrophobic binding of the molecule with its lipid anchor to the dextran matrix. Alternatively, the protein was fixed in a similar orientation by covalent linkage through a perfluorophenylazide-derived… CONTINUE READING