Orientation of cobra alpha-toxin on the nicotinic acetylcholine receptor. Fluorescence studies.

@article{Johnson1990OrientationOC,
  title={Orientation of cobra alpha-toxin on the nicotinic acetylcholine receptor. Fluorescence studies.},
  author={David S. Johnson and Richard Cushman and Reza Malekzadeh},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 13},
  pages={7360-8}
}
Four flourescein isothiocyanate (FITC) derivatives of Naja naja siamemsis 3 neurotoxin (alpha-toxin), labeled at the epsilon-amino groups of Lys-23, Lys-35, Lys-49, or Lys-69, and a tetramethylrhodamine isothiocyanate (TRITC) derivative, labeled at epsilon-amino group of Lys-23, were prepared and used to analyze the orientation of cobra alpha-toxin on the nicotinic acetylcholine receptor (AcChR) relative to both the plane of the membrane and the central ion channel. Fluorescence-quenching… CONTINUE READING