Orientation and dynamics of a novel fluorescent cholesterol analogue in membranes of varying phase.

@article{Shrivastava2009OrientationAD,
  title={Orientation and dynamics of a novel fluorescent cholesterol analogue in membranes of varying phase.},
  author={Sandeep Shrivastava and Sourav Haldar and Gerald Gimpl and Amitabha Chattopadhyay},
  journal={The journal of physical chemistry. B},
  year={2009},
  volume={113 13},
  pages={
          4475-81
        }
}
Fluorescent analogues of cholesterol offer a powerful approach for monitoring cholesterol behavior in membranes because of their high sensitivity, suitable time resolution, and multiplicity of measurable parameters. In this work, we have monitored the orientation and dynamics of a novel fluorescent cholesterol probe, 6-dansylcholestanol (DChol), in membranes of different phase type utilizing sensitive fluorescence techniques including the red-edge excitation shift (REES) approach. Our results… 

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This book describes the fundamental aspects of fluorescence, the biochemical applications of this methodology, and the instrumentation used in fluorescence spectroscopy.