Organotypic cortex-striatum-mesencephalon cultures: the nigrostriatal pathway.

@article{Plenz1996OrganotypicCC,
  title={Organotypic cortex-striatum-mesencephalon cultures: the nigrostriatal pathway.},
  author={Dietmar Plenz and Stephen T. Kitai},
  journal={Neuroscience letters},
  year={1996},
  volume={209 3},
  pages={177-80}
}
We were successful in developing cortex-striatum-mesencephalon organotypic cultures from the rat brain after 4-9 weeks in vitro. A modification of the 'roller tube' technique was employed where slices were embedded in a plasma/thrombin clot onto a Millicell membrane on a coverslip. The underlying membrane provided high mechanical stability during culturing… CONTINUE READING