Organotypic 3D cell culture models: using the rotating wall vessel to study host-pathogen interactions.

Abstract

Appropriately simulating the three-dimensional (3D) environment in which tissues normally develop and function is crucial for engineering in vitro models that can be used for the meaningful dissection of host-pathogen interactions. This Review highlights how the rotating wall vessel bioreactor has been used to establish 3D hierarchical models that range in complexity from a single cell type to multicellular co-culture models that recapitulate the 3D architecture of tissues observed in vivo. The application of these models to the study of infectious diseases is discussed.

DOI: 10.1038/nrmicro2423
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@article{Barrila2010Organotypic3C, title={Organotypic 3D cell culture models: using the rotating wall vessel to study host-pathogen interactions.}, author={Jennifer Barrila and Andrea L. Radtke and Aur{\'e}lie Crabb{\'e} and Shameema F. Sarker and Melissa M Herbst-Kralovetz and Charlie M Ott and Cheryl A Nickerson}, journal={Nature reviews. Microbiology}, year={2010}, volume={8 11}, pages={791-801} }