Organotypic 3D cell culture models: using the rotating wall vessel to study host–pathogen interactions

@article{Barrila2010Organotypic3C,
  title={Organotypic 3D cell culture models: using the rotating wall vessel to study host–pathogen interactions},
  author={Jennifer Barrila and Andrea Lynn Radtke and Aur{\'e}lie Crabb{\'e} and Shameema F. Sarker and Melissa M. Herbst-Kralovetz and Charlie Mark Ott and Cheryl A. Nickerson},
  journal={Nature Reviews Microbiology},
  year={2010},
  volume={8},
  pages={791-801}
}
Appropriately simulating the three-dimensional (3D) environment in which tissues normally develop and function is crucial for engineering in vitro models that can be used for the meaningful dissection of host–pathogen interactions. This Review highlights how the rotating wall vessel bioreactor has been used to establish 3D hierarchical models that range in complexity from a single cell type to multicellular co-culture models that recapitulate the 3D architecture of tissues observed in vivo. The… 
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234 Citations
Highly Influential Citations
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Background Citations
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Methods Citations
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234 Citations

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TLDR
The goal is to present a non-conventional means of culturing human epithelial cells to produce organotypic 3-D models that recapitulate the human in vivo tissue, in a facile and robust system to be used by researchers with diverse scientific interests.
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RWV-derived 3-D tissue culture models mimic key aspects of the differentiated structure and function of their respective in vivo parental tissues, and are attractive platforms to help bridge the gap between cell-based discoveries at the bench, and clinical translation.
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This review summarizes and analyzes recent progress in human virological research with 3D cell culture models and discusses viral growth, replication, proliferation, infection, virus-host interactions and antiviral drugs in 3D culture models.
Therapeutic strategies of three-dimensional stem cell spheroids and organoids for tissue repair and regeneration
Modelling Toxoplasma gondii infection in a 3D cell culture system In Vitro: Comparison with infection in 2D cell monolayers
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The utility of collagen matrices for studying parasite modes of infection as these 3D assays more closely mimic in vivo conditions is demonstrated.
From Single Cells to Engineered and Explanted Tissues: New Perspectives in Bacterial Infection Biology.
A microfabricated platform to form three-dimensional toroidal multicellular aggregate
TLDR
This work has developed a unique multicellular aggregate formation platform that utilizes a maskless gray-scale photolithography and has a toroidal-like geometry and includes a micro lumen that facilitates the supply of oxygen and growth factors and the expulsion of waste products.
Multiscale assembly for tissue engineering and regenerative medicine.
3D tissue-like assemblies: A novel approach to investigate virus–cell interactions
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