Organotypic 3D cell culture models: using the rotating wall vessel to study host–pathogen interactions

  title={Organotypic 3D cell culture models: using the rotating wall vessel to study host–pathogen interactions},
  author={Jennifer Barrila and Andrea Lynn Radtke and Aur{\'e}lie Crabb{\'e} and Shameema F. Sarker and Melissa M. Herbst-Kralovetz and Charlie Mark Ott and Cheryl A. Nickerson},
  journal={Nature Reviews Microbiology},
Appropriately simulating the three-dimensional (3D) environment in which tissues normally develop and function is crucial for engineering in vitro models that can be used for the meaningful dissection of host–pathogen interactions. This Review highlights how the rotating wall vessel bioreactor has been used to establish 3D hierarchical models that range in complexity from a single cell type to multicellular co-culture models that recapitulate the 3D architecture of tissues observed in vivo. The… 
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