Organophosphorus hydrolase is a remarkably stable enzyme that unfolds through a homodimeric intermediate.

@article{Grimsley1997OrganophosphorusHI,
  title={Organophosphorus hydrolase is a remarkably stable enzyme that unfolds through a homodimeric intermediate.},
  author={Janet K Grimsley and J. Martin Scholtz and C. Nick Pace and James R. Wild},
  journal={Biochemistry},
  year={1997},
  volume={36 47},
  pages={14366-74}
}
Organophosphorus hydrolase (OPH, EC 8.1.3.1) is a homodimeric enzyme that catalyzes the hydrolysis of organophosphorus pesticides and nerve agents. We have analyzed the urea- and guanidinium chloride-induced equilibrium unfolding of OPH as monitored by far-ultraviolet circular dichroism and intrinsic tryptophan fluorescence. These spectral methods, which monitor primarily the disruption of protein secondary structure and tertiary structure, respectively, reveal biphasic unfolding transitions… CONTINUE READING

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