Organization of the multifunctional enzyme type 1: interaction between N- and C-terminal domains is required for the hydratase-1/isomerase activity.

@article{Kiema2002OrganizationOT,
  title={Organization of the multifunctional enzyme type 1: interaction between N- and C-terminal domains is required for the hydratase-1/isomerase activity.},
  author={T. Kiema and Jukka P Taskinen and P{\"a}ivi L. Piril{\"a} and Kari T Koivuranta and Rik Wierenga and J Kalervo Hiltunen},
  journal={The Biochemical journal},
  year={2002},
  volume={367 Pt 2},
  pages={433-41}
}
Rat peroxisomal multifunctional enzyme type 1 (perMFE-1) is a monomeric protein of beta-oxidation. We have defined five functional domains (A, B, C, D and E) in the perMFE-1 based on comparison of the amino acid sequence with homologous proteins from databases and structural data of the hydratase-1/isomerases (H1/I) and (3 S )-hydroxyacyl-CoA dehydrogenases (HAD). Domain A (residues 1-190) comprises the H1/I fold and catalyses both 2-enoyl-CoA hydratase-1 and Delta(3)-Delta(2)-enoyl-CoA… CONTINUE READING

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