Organization of the cis-acting element required for wheat dwarf geminivirus DNA replication and visualization of a rep protein-DNA complex.

@article{SanzBurgos1998OrganizationOT,
  title={Organization of the cis-acting element required for wheat dwarf geminivirus DNA replication and visualization of a rep protein-DNA complex.},
  author={Andr{\'e}s P. Sanz-Burgos and Crisanto Gutierrez},
  journal={Virology},
  year={1998},
  volume={243 1},
  pages={119-29}
}
Initiation of geminivirus DNA replication depends on the activity of the initiator protein (Rep) upon interaction with DNA sequences present in the intergenic region of the viral DNA. In this study, we have analyzed the DNA sequences present in the large intergenic region (LIR) of wheat dwarf virus (WDV), a subgroup I member of the geminivirus family, which are required for viral DNA replication. We have (i) defined the boundaries of the viral cis-acting DNA replication element, (ii) determined… CONTINUE READING
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