For a long period of time two factors have impeded investigations of myxosporidia physiology: the small size of their nuclei and the absence of data about existence of condensed chromosomes in these organisms. Significant progress in biological research methods provides new approaches to investigations of Myxozoa chromatin and chromosomal apparatus. Using confocal scanning microscopy chromosomes in the generative cell nuclei of Zschokkela nova (Klokacewa, 1914) were revealed and described in this work for the first time. It has been shown that their karyotype consists of six chromosomes (three pairs). Two pairs of chromosomes have an oblong rod-shaped form. Chromosomes of the third pair have a boomerang form (with a bend in the middle of the chromosomes body and with a thickening on one of the ends). Lengths of chromosomes: 5 microm (1st pair), 4.8 microm (2nd pair) and 3 microm (3rd pair). The culture of plasmodium cells Myxobolus pseudodispar (Gorbunova, 1936) was received.