Organization of a cluster of erythromycin genes in Saccharopolyspora erythraea

@article{Weber1990OrganizationOA,
  title={Organization of a cluster of erythromycin genes in Saccharopolyspora erythraea},
  author={J. Mark Weber and J O Leung and Gregory T. Maine and R. Potenz and Thomas J. Paulus and Judy Park DeWitt},
  journal={Journal of Bacteriology},
  year={1990},
  volume={172},
  pages={2372 - 2383}
}
We used a series of gene disruptions and gene replacements to mutagenically characterize 30 kilobases of DNA in the erythromycin resistance gene (ermE) region of the Saccharopolyspora erythraea chromosome. Five previously undiscovered loci involved in the biosynthesis of erythromycin were found, eryBI, eryBII, eryCI, eryCII, and eryH; and three known loci, eryAI, eryG, and ermE, were further characterized. The new Ery phenotype, EryH, was marked by (i) the accumulation of the intermediate 6… 

Figures and Tables from this paper

The erythromycin biosynthetic gene cluster of Aeromicrobium erythreum
TLDR
The gene order of the two ery clusters was conserved within a core region of 15 contiguous genes, with the exception of IS1136 which was not found in the A. erythreum cluster.
Analysis of eryBI, eryBIII and eryBVII from the erythromycin biosynthetic gene cluster in Saccharopolyspora erythraea
TLDR
Specific mutants were created in eryBI, and the resulting strains were found to synthesise erythromycin A, showing that this gene, despite its position in the biosynthetic gene cluster, is not essential for erystromycin biosynthesis.
Analysis of seven genes from the eryAI –eryK region of the erythromycin biosynthetic gene cluster in Saccharopolyspora erythraea
TLDR
R roles are proposed for several of these ORFs in the biosynthesis of the deoxysugar mycarose and the deoxyaminosugar desosamine in the macrolide antibiotic erythromycin A by Saccharopolyspora erystraea.
Targeted gene inactivation for the elucidation of deoxysugar biosynthesis in the erythromycin producer Saccharopolyspora erythraea
TLDR
The nucleotide sequence of three such ORFs located immediately downstream of eryA, ORFs 7, 8 and 9 are reported, which considerably strengthen previous tentative proposals for the pathways for the biosynthesis of dTDP-D-desosamine and dTSP-L-mycarose in Sac.
Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases
TLDR
Comparison of the amino acid sequence of EryG with the sequence of other S-adenosylmethionine (SAM)-dependent methyltransferases has revealed that one of the sequence motifs previously suggested to be part of the SAM-binding site is present not only in EyG but also in many other recently sequenced SAM-dependent methyl transferases.
Transcriptional Organization of the Erythromycin Biosynthetic Gene Cluster of Saccharopolyspora erythraea
TLDR
The results demonstrate that the ery gene cluster contains four major polycistronic transcriptional units, the largest one extending approximately 35 kb from eryAI to eryG.
Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis
TLDR
A gene, designated eryK, located about 50 kb downstream of the erythromycin resistance gene, ermE, encodes a 44-kDa protein which, on the basis of comparative analysis, belongs to the P450 monooxygenase family.
Dissecting and engineering of the TetR family regulator SACE_7301 for enhanced erythromycin production in Saccharopolyspora erythraea
TLDR
This study markedly improves the understanding of the unusual regulatory mechanism of erythromycin biosynthesis, and provides a novel strategy towards Er-A overproduction by engineering transcriptional regulators of S. eryTHraea.
...
...

References

SHOWING 1-10 OF 40 REFERENCES
Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus)
Genes that govern the formation of deoxysugars or their attachment to erythronolide B and 3 alpha-mycarosyl erythronolide B, intermediates of the biosynthesis of the 14-membered macrolide antibiotic
Molecular characterization of a gene from Saccharopolyspora erythraea (Streptomyces erythraeus) which is involved in erythromycin biosynthesis
TLDR
The eryC1‐gene product, a protein of subunit Mr 39200, is therefore involved either as a structural or as a regulatory gene in the formation of the deoxyamino‐sugar desosamine or in its attachment to the macro‐lide ring.
Genetic analysis of erythromycin production in Streptomyces erythreus
TLDR
Demonstration of the occurrence of natural genetic recombination during conjugal mating in S. erythreus enabled comparison of the genetic linkage relationships of three different ery mutations with seven other markers on a simple chromosome map.
Mutation and cloning of eryG, the structural gene for erythromycin O-methyltransferase from Saccharopolyspora erythraea, and expression of eryG in Escherichia coli
TLDR
It appears that a single enzyme catalyzes all of the 3"-O-methylation reactions of the erythromycin biosynthetic pathway in S. erystraea and that eryG codes for the structural gene of this enzyme.
Biochemical Mechanisms in the Biosynthesis of the Erythromycins
TLDR
This chapter analyzes in detail the present state of knowledge concerning the biosynthesis of the erythromycins and presents and discusses some general aspects of biosynthesis with this model system as an example.
Macrolide antibiotic biosynthesis: isolation and properties of two forms of 6-deoxyerythronolide B hydroxylase from Saccharopolyspora erythraea (Streptomyces erythreus).
TLDR
A cytochrome P-450 monooxygenase that catalyzes the hydroxylation of 6-deoxyerythronolide B, an intermediate of erythromycin A biosynthesis in Saccharopolyspora erystraea, was resolved into two forms by hydroxymapatite chromatography and showed identical antigenicity and cross-reactivity against polyvalent and specific antibodies.
Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.
TLDR
PIJ702 is a useful cloning vector with insertional inactivation of the Mel+ character as the basis of clone recognition and Restriction mapping of the tyrosinase fragment in pIJ702 revealed endonuclease cleavage sites for several enzymes, including single sites for BglII, SphI and SstI that are absent from the parent vector.
...
...