Organization of a cluster of erythromycin genes in Saccharopolyspora erythraea

  title={Organization of a cluster of erythromycin genes in Saccharopolyspora erythraea},
  author={J. Mark Weber and J O Leung and Gregory T. Maine and R. Potenz and Thomas J. Paulus and Judy Park DeWitt},
  journal={Journal of Bacteriology},
  pages={2372 - 2383}
We used a series of gene disruptions and gene replacements to mutagenically characterize 30 kilobases of DNA in the erythromycin resistance gene (ermE) region of the Saccharopolyspora erythraea chromosome. Five previously undiscovered loci involved in the biosynthesis of erythromycin were found, eryBI, eryBII, eryCI, eryCII, and eryH; and three known loci, eryAI, eryG, and ermE, were further characterized. The new Ery phenotype, EryH, was marked by (i) the accumulation of the intermediate 6… 

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