Organization of Escherichia coli O157 O Antigen Gene Cluster and Identification of Its Specific Genes

  title={Organization of Escherichia coli O157 O Antigen Gene Cluster and Identification of Its Specific Genes},
  author={Lei Wang and Peter R. Reeves},
  journal={Infection and Immunity},
  pages={3545 - 3551}
ABSTRACT The O157:H7 clone of Escherichia coli, which causes major, often prolonged outbreaks of gastroenteritis with hemolytic-uremic syndrome (HUS) such as those in Japan, Scotland, and the United States recently, is thought to be resident normally in cattle or other domestic animals. This clone is of major significance for public health and the food industry. We have developed a fast method for sequencing a given O antigen gene cluster and applied it to O157. The O157 O antigen gene cluster… 

Sequencing of Escherichia coli O111 O-Antigen Gene Cluster and Identification of O111-Specific Genes

This work provides the basis for a sensitive test for the rapid detection of E. coli O111, one of the most frequently isolated non-O157 strains causing outbreaks of gastroenteritis with hemolytic-uremic syndrome.

Identification of Escherichia coli O114 O-Antigen Gene Cluster and Development of an O114 Serogroup-Specific PCR Assay

The PCR assays established in this study can be used to reliably identify E. coli O114 strains and may also be used in food, water, and other environmental samples and shown to be highly specific and sensitive.

Association of Nucleotide Polymorphisms within the O-Antigen Gene Cluster of Escherichia coli O26, O45, O103, O111, O121, and O145 with Serogroups and Genetic Subtypes

The SNPs discovered in this study can be used to develop tests that will not only accurately identify O26, O45, O103, O111, O121, and O145 strains but also predict whether strains detected in the above-described serogroups contain Shiga toxin-encoding genes.

DNA sequence of the Escherichia coli O103 O antigen gene cluster and detection of enterohemorrhagic E. coli O103 by PCR amplification of the wzx and wzy genes.

The DNA sequence of the 12,033-bp region containing the O antigen gene cluster of Escherichia coli O103 was determined and PCR-based methods for detection and identification of this serogroup were developed.

The Escherichia coli O111 and Salmonella enterica O35 Gene Clusters: Gene Clusters Encoding the Same Colitose-Containing O Antigen Are Highly Conserved

It is concluded that the ancestor of E. coli and S. enterica had an O antigen identical to the O111 and O35 antigens, respectively, of these species and that the gene cluster encoding it has survived in both species.

Sequence of the Escherichia coli O121 O-Antigen Gene Cluster and Detection of Enterohemorrhagic E. coli O121 by PCR Amplification of the wzx and wzy Genes

The PCR assay can be employed to reliably identify E. coli O121 and to potentially detect the organism in food, fecal, and environmental samples.



Clonal relationships among Escherichia coli strains that cause hemorrhagic colitis and infantile diarrhea

It is proposed that the new pathogen emerged when an O55:H7-like progenitor, already possessing a mechanism for adherence to intestinal cells, acquired secondary virulence factors (Shiga-like cytotoxins and plasmid-encoded adhesins) via horizontal transfer and recombination.

Genetic relationships among pathogenic Escherichia coli of serogroup O157

The results indicate that O157 E. coli are genetically diverse and strongly suggest that the O157:H7 lineage was not recently derived from other pathogenic strains of the O 157 serogroup.

Role of the Escherichia coli O157:H7 O side chain in adherence and analysis of an rfb locus

It is concluded that rfbE(EcO157:H7) is necessary for the expression of the O157 antigen, that acquisition of E. coli rfb genes occurred independently in E. Escherichia coli O157: H7 and unrelated O157 strains, and that the O side chain ofE.

Escherichia coli O157:H7: clinical, diagnostic, and epidemiological aspects of human infection.

  • P. Tarr
  • Medicine
    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
  • 1995
Research should be directed at reducing the carriage of E. coli O157:H7 at its bovine source, minimizing the microbial content of food and water, and averting systemic microangiopathic hemolytic anemia after infection with this pathogen.

Evolutionary relationships among pathogenic and nonpathogenic Escherichia coli strains inferred from multilocus enzyme electrophoresis and mdh sequence studies

The results suggest the possibility that any E. coli strain acquiring the appropriate virulence factors may give rise to a pathogenic form, and that the genus Shigella is shown to comprise a group of closely related pathogenic E. Escherichia coli strains.

Detection of Escherichia coli O157:H7 by multiplex PCR

The multiplex PCR method can be used to specifically identify EHEC of serogroup O157, which is known to contain Shiga-like toxins I and II and to contain the 60-MDa plasmid of E. coli O157.

Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid

The sequence of a 23-kb segment of the E. coli K-12 chromosome is determined which includes the cluster of genes necessary for production of CA, and the first three genes of the cluster are predicted to encode an outer membrane lipoprotein, a phosphatase, and an inner membrane protein with an ATP-binding domain.

Molecular analysis of the rfb gene cluster of a group D2 Salmonella enterica strain: evidence for its origin from an insertion sequence-mediated recombination event between group E and D1 strains

Mapping, hybridization, and DNA sequencing showed that the organization of the D2 rfb genes is similar to that of group D1, with the alpha-mannosyl transferase gene rfbU replaced by rfbO, the E1-specific beta-mannOSyl transfer enzyme gene.

Characterization of the rfc region of Shigella flexneri

The isolated S. flexneri mutants are deficient in production of the O-antigen polymerase and were termed rfc mutants, and Examination of the distribution of rare or major codons in the rfc gene revealed that it has several minor codons within the first 25 amino acids, in contrast to the upstream gene rfbG, which has a high percentage of rare codons but whose gene product could be detected.