Organization of Escherichia coli O157 O Antigen Gene Cluster and Identification of Its Specific Genes

@article{Wang1998OrganizationOE,
  title={Organization of Escherichia coli O157 O Antigen Gene Cluster and Identification of Its Specific Genes},
  author={Lei Wang and Peter R Reeves},
  journal={Infection and Immunity},
  year={1998},
  volume={66},
  pages={3545 - 3551}
}
  • Lei Wang, P. Reeves
  • Published 1998
  • Biology, Medicine
  • Infection and Immunity
ABSTRACT The O157:H7 clone of Escherichia coli, which causes major, often prolonged outbreaks of gastroenteritis with hemolytic-uremic syndrome (HUS) such as those in Japan, Scotland, and the United States recently, is thought to be resident normally in cattle or other domestic animals. This clone is of major significance for public health and the food industry. We have developed a fast method for sequencing a given O antigen gene cluster and applied it to O157. The O157 O antigen gene cluster… Expand
Sequencing of Escherichia coli O111 O-Antigen Gene Cluster and Identification of O111-Specific Genes
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This work provides the basis for a sensitive test for the rapid detection of E. coli O111, one of the most frequently isolated non-O157 strains causing outbreaks of gastroenteritis with hemolytic-uremic syndrome. Expand
Sequence of the Escherichia coli O26 O antigen gene cluster and identification of O26 specific genes.
TLDR
This work identified three O antigen genes that are highly specific to O26 that provide the basis for a sensitive test for the rapid detection of pathogenic clones with the O26 antigen, which has implications for public health, especially in the control of food-borne outbreaks. Expand
Characterization of Escherichia coli O86 O-antigen gene cluster and identification of O86-specific genes.
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A polymerase chain reaction (PCR) assay, based on the specific O-antigen genes identified here, could be used for the rapid detection of E. coli O86 in environmental and clinical samples. Expand
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Identification of Escherichia coli O114 O-Antigen Gene Cluster and Development of an O114 Serogroup-Specific PCR Assay
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Association of Nucleotide Polymorphisms within the O-Antigen Gene Cluster of Escherichia coli O26, O45, O103, O111, O121, and O145 with Serogroups and Genetic Subtypes
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DNA sequence of the Escherichia coli O103 O antigen gene cluster and detection of enterohemorrhagic E. coli O103 by PCR amplification of the wzx and wzy genes.
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The DNA sequence of the 12,033-bp region containing the O antigen gene cluster of Escherichia coli O103 was determined and PCR-based methods for detection and identification of this serogroup were developed. Expand
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TLDR
In this study, the O antigen gene clusters of E. coli O3 and O21 were characterized, and PCR assays based on O antigen specific genes wzx and wzy from each strain were developed and shown to be highly specific to O3 or O21 respectively. Expand
The Escherichia coli O111 and Salmonella enterica O35 gene clusters: gene clusters encoding the same colitose-containing O antigen are highly conserved.
  • Lei Wang, P. Reeves
  • Medicine, Biology
  • Journal of bacteriology
  • 2000
TLDR
It is concluded that the ancestor of E. coli and S. enterica had an O antigen identical to the O111 and O35 antigens, respectively, of these species and that the gene cluster encoding it has survived in both species. Expand
Sequence of the Escherichia coli O121 O-Antigen Gene Cluster and Detection of Enterohemorrhagic E. coli O121 by PCR Amplification of the wzx and wzy Genes
TLDR
The PCR assay can be employed to reliably identify E. coli O121 and to potentially detect the organism in food, fecal, and environmental samples. Expand
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