Organization and topology of photosystem I subunits.

@article{Zilber1992OrganizationAT,
  title={Organization and topology of photosystem I subunits.},
  author={A L Zilber and Richard Malkin},
  journal={Plant physiology},
  year={1992},
  volume={99 3},
  pages={901-11}
}
Intact spinach (Spinacia oleracea) thylakoid membranes were treated with various proteases and photosystem I (PSI) complexes were isolated from these membranes to define the membrane topology of specific PSI subunits. Trypsin treatment caused cleavage of the PSI-D and E subunits. Thermolysin treatment cleaved the PSI-D, E, H, and K subunits, and also caused limited degradation of the reaction center core PSI-A and B subunits. Pronase treatment produced the most dramatic results as the PSI-A and… CONTINUE READING

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