Organisation of the murine 5‐HT3 receptor gene and assignment tohuman chromosome 11

  title={Organisation of the murine 5‐HT3 receptor gene and assignment tohuman chromosome 11},
  author={P. Uetz and F Abdelatty and Alfredo Villarroel and Gudrun A. Rappold and Birgit Weiss and Michael Koenen},
  journal={FEBS Letters},
Developmental Changes in the Differential Expression of Two Serotonin 5‐HT3 Receptor Splice Variants in the Rat
Abstract: PCR was used to isolate identical partial cDNA clones encoding a serotonin 5‐HT3 receptor subunit from rat nodose and superior cervical ganglia. The amino acid sequence predicted from these
Evidence for Expression of Heteromeric Serotonin 5‐HT3 Receptors in Rodents
Functional data and expression patterns are consistent with the existence of heteromeric 5‐HT3 receptors in rodent neurons, and sequence analysis and functional studies suggest that the new rodent subunits are orthologues of the human 5-HT3B subunit.
The human glycine receptor subunit alpha3. Glra3 gene structure, chromosomal localization, and functional characterization of alternative transcripts.
Functional expression in HEK 293 cells of alpha3L and alpha3K subunits resulted in the formation of glycine-gated chloride channels that differed significantly in desensitization behavior, thus defining the cytoplasmic loop as an important determinant of channel inactivation kinetics.
Chromosomal localization of 15 ion channel genes
The ESTs developed in this study can be used to isolate genomic clones, enabling the determination of each transcript's genomic structure and physical map location, and may aid in the identification of candidate genes for groups of related heritable disorders.
Functional characterization of two 5-HT3 receptor splice variants isolated from a mouse hippocampal cell line
Two splice variants of the ligand-gated 5-hydroxytryptamine or serotonin 5-HT3 receptor that differ in a six-amino-acid deletion were cloned by polymerase chain reaction from the hippocampus x neuroblastoma cell line HN9.10e to reveal no significant differences in current responses to the agonists 5- HT and 1-phenylbiguanide.
Molecular cloning, functional expression, and pharmacological characterization of 5-hydroxytryptamine3 receptor cDNA and its splice variants from guinea pig.
The data reveal that the cloned cDNAs code for guinea pig 5-HT3 receptors, which functionally assemble as homo-oligomers, and the kinetic behavior of the ion channel and its sensitivity to several agonists and antagonists were markedly different from those of theCloned 5- HT3 receptors from mouse and human under similar experimental conditions.
The Serotonin Receptor Subtype 5b Specifically Interacts with Serotonin Receptor Subtype 1A
It is found that 5-ht5b receptors can specifically interact with 5-HT1A receptors and retain them in endosomal compartments, which possibly represents a mechanism by which 4 and fifth trans-membrane domain (TMD) regulates the activity of other 5- HT receptor.


The Murine GABAA Receptor δ-Subunit Gene: Structure and Assignment to Human Chromosome 1
The murine chromosomal gene for the GABAA receptor delta subunit was isolated and characterized by high-resolution mapping and DNA sequencing and displays an intron pattern comparable, but not identical, to that seen in members of the nicotinic acetylcholine receptor family.
Genes expressed in the brain define three distinct neuronal nicotinic acetylcholine receptors.
Four genes encode the related protein subunits that assemble to form the nicotinic acetylcholine receptor (nAChR) at the motor endplate of vertebrates and probably exists in all vertebrates since their counterparts have also been identified in the rat genome.
Conserved Organization of γ‐Aminobutyric AcidAReceptor Genes: Cloning and Analysis of the Chicken β4‐Subunit Gene
A series of genomic clones containing DNA that encodes the chicken GABAA receptor β4 subunit have been isolated to determine the structural organization and the size of the β4‐subunit gene, and the proposal that this superfamily of ligand‐gated ion‐channel receptor genes arose by duplication of an ancestral receptor gene is discussed.
Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel.
A complementary DNA clone containing the coding sequence of one of these rapidly responding channels, a 5HT3 subtype of the serotonin receptor, has been isolated by screening a neuroblastoma expression library for functional expression of serotonin-gated currents in Xenopus oocytes.
Drosophila and vertebrate myb proteins share two conserved regions, one of which functions as a DNA‐binding domain.
DNA‐binding experiments indicate that the NH2‐proximal of the two homology regions functions as a DNA‐binding domain, and it is likely that this homology region encodes a function whose loss is involved in activating the oncogenic potential of c‐myb.
Molecular biology of GABAA receptors
  • R. Olsen, A. Tobin
  • Biology, Chemistry
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 1990
Subpopulations of GABAA receptors with different cellular and regional locations show differential sensitivity to GABA, to modulators like steroids, to physiological regulation, to disease processes, and to pharmacological manipulation by drugs such as benzodiazepines.
Functional expression of a new pharmacological subtype of brain nicotinic acetylcholine receptor.
Results demonstrate that the alpha 2 subunit of rat neuronal nicotinic acetylcholine receptors differs from other known agonist-binding alpha-subunits of nAChRs in its distribution in the brain and in its pharmacology.
Cloning and sequence analysis of calf cDNA and human genomic DNA encoding α-subunit precursor of muscle acetylcholine receptor
Nucleotide sequence analysis of the cloned DNAs has revealed the primary structures of the calf and human AChR α-subunit precursors, which exhibit marked sequence homology with their Torpedo counterpart.