Organelle DNA analysis of Solanum and Brassica somatic hybrids by PCR with ’universal primers’

  title={Organelle DNA analysis of Solanum and Brassica somatic hybrids by PCR with ’universal primers’},
  author={Tanja Bastia and Nunzia Scotti and Teodoro Cardi},
  journal={Theoretical and Applied Genetics},
In order to set up a quick and easy procedure for determining the cytoplasmic composition of somatic hybrids, we tested a set of ’universal primers’ for plastidial and mitochondrial DNA on 13 genotypes belonging to the following species: Nicotiana tabacum, Solanum commersonii, Solanum tuberosum, Solanum etuberosum, Solanum phureja, Brassica oleracea, Brassica rapa, ’Anand’ CMS B. rapa, ’Chiang’ CMS B. oleracea, and ’Ogura’ CMS B. oleracea. Such primers are homologous to conserved coding… CONTINUE READING
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