Ordered restriction maps of Saccharomyces cerevisiae chromosomes constructed by optical mapping.

@article{Schwartz1993OrderedRM,
  title={Ordered restriction maps of Saccharomyces cerevisiae chromosomes constructed by optical mapping.},
  author={David C. Schwartz and Xianran Li and Lisa Hernandez and Shakuntala Ramnarain and Edward J. Huff and Y K Wang},
  journal={Science},
  year={1993},
  volume={262 5130},
  pages={110-4}
}
A light microscope-based technique for rapidly constructing ordered physical maps of chromosomes has been developed. Restriction enzyme digestion of elongated individual DNA molecules (about 0.2 to 1.0 megabases in size) was imaged by fluorescence microscopy after fixation in agarose gel. The size of the resulting individual restriction fragments was… CONTINUE READING