Oral platelet aggregation inhibitor Ro 48-3657: determination of the active metabolite and its prodrug in plasma and urine by high-performance liquid chromatography using automated column switching.

@article{Timm1997OralPA,
  title={Oral platelet aggregation inhibitor Ro 48-3657: determination of the active metabolite and its prodrug in plasma and urine by high-performance liquid chromatography using automated column switching.},
  author={Uwe Timm and R. Zumbrunnen and Robert Erdin and Manfred Singer and Bruce F Steiner},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={1997},
  volume={691 2},
  pages={397-407}
}
A sensitive and highly automated high-performance liquid chromatography (HPLC) column-switching method has been developed for the simultaneous determination of the active metabolite III and its prodrug II, both derivatives of the oral platelet inhibitor Ro 48-3657 (I), in plasma and urine of man and dog. Plasma samples were deproteinated with perchloric acid (0.5 M), while urine samples could be processed directly after dilution with phosphate buffer. The prepared samples were injected onto a… CONTINUE READING