Orai-STIM-mediated Ca2+ release from secretory granules revealed by a targeted Ca2+ and pH probe.


Secretory granules (SGs) sequester significant calcium. Understanding roles for this calcium and potential mechanisms of release is hampered by the difficulty of measuring SG calcium directly in living cells. We adapted the Förster resonance energy transfer-based D1-endoplasmic reticulum (ER) probe to develop a unique probe (D1-SG) to measure calcium and pH… (More)
DOI: 10.1073/pnas.1218247109


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