Optogenetics: controlling cell function with light

  title={Optogenetics: controlling cell function with light},
  author={Erika Pastrana},
  journal={Nature Methods},
A brief description of the basic steps required to control cellular function with optogenetics is presented. 
Prospects for Therapeutic Application of Optogenetics
The prospects for the therapeutic applications of optogenetics using the channelrhodopsin 2, which is a typical light receptor, and the recent research results are summarized. Expand
Nitrobenzyl-based fluorescent photocages for spatial and temporal control of signalling lipids in cells.
A set of fluorescent cages prepared by tethering fluorescent dyes to a photolabile group enable caging of signalling lipids, their delivery to specific cellular membranes, with further imaging, quantification, and controlled photorelease of active lipids in living cells. Expand
Nanoscale Optomechanical Actuators for Controlling Mechanotransduction in Living Cells
  • Z. Liu, Yang Liu, +7 authors K. Salaita
  • Materials Science, Medicine
  • Nature Methods
  • 2016
To control receptor tension optically at the cell surface, an approach involving optomechanical actuator nanoparticles that are controlled with near-infrared light is developed, controlling integrin-based focal adhesion formation, cell protrusion and migration, and T cell receptor activation. Expand
Suppression of the Spectral Cross Talk of Optogenetic Switching by Stimulated Depletion Quenching. Theoretical Analysis.
This work proposes and demonstrates through simulations a new control approach of Stimulated Depletion Quenching, applied to the phytochrome Cph8 bidirectional optogenetic switch, and the results show significant improvement of its dynamic range. Expand
Optical techniques in optogenetics
Basic concepts that are essential for a comprehensive understanding of optogenetics and some important biological/biomedical applications are highlighted. Expand
Current Topics of Optogenetics for Medical Applications Toward Therapy.
  • T. Kushibiki
  • Medicine
  • Advances in experimental medicine and biology
  • 2021
Current reports on therapy using a typical photopigment used in optogenetics, channelrhodopsin-2, are summarized. Expand
Reversible spatial and temporal control of lipid signaling.
Versatile molecular tools that enable specific delivery and visualization of photoswitchable lipids at cellular membranes, namely at the plasma membrane and internal membranes are introduced. Expand
Optogenetics: Novel Tools for Controlling Mammalian Cell Functions with Light
The photosensitive channel proteins involved in optogenetics are described, techniques for their targeting to neurons and other cell types both within and outside the nervous system, and their applications in the field of neuroscience and beyond are discussed. Expand
Optogenetic Technology and Its In Vivo Applications
Progress in the in vivo applications of optogenetics is reviewed in this article, with particular focus on behavioral applications in nematodes, fish, rodents, and nonhuman primates. Expand
General Description: Future Prospects of Optogenetics
In this chapter, the topics and the prospects of optogenetics are discussed from three view points: the optimization of OMRs, the Optimization of gene-targeting methods, and the optimized of optics. Expand