Optogenetic probing of functional brain circuitry
@article{Mancuso2011OptogeneticPO, title={Optogenetic probing of functional brain circuitry}, author={James J. Mancuso and Jinsook Kim and Soojung Lee and Sachiko Tsuda and Nicholas Boon-How Chow and George J. Augustine}, journal={Experimental Physiology}, year={2011}, volume={96} }
Recently developed optogenetic technologies offer the promise of high‐speed mapping of brain circuitry. Genetically targeted light‐gated channels and pumps, such as channelrhodopsins and halorhodopsin, allow optical control of neuronal activity with high spatial and temporal resolution. Optogenetic probes of neuronal activity, such as Clomeleon and Mermaid, allow light to be used to monitor the activity of a genetically defined population of neurons. Combining these two complementary sets of…
59 Citations
Optogenetic mapping of brain circuitry
- BiologyOther Conferences
- 2012
This work has mapped the circuitry of the mouse brain by using both optogenetic actuators that control neuronal activity andoptogenetic sensors that detect neuronal activity to create unprecedented opportunities to explore brain function, screen pharmaceutical agents, and potentially to use light to ameliorate psychiatric and neurological disorders.
Probing the function of neuronal populations: Combining micromirror-based optogenetic photostimulation with voltage-sensitive dye imaging
- BiologyNeuroscience Research
- 2013
High-speed optogenetic circuit mapping
- BiologyPhotonics West - Biomedical Optics
- 2013
ChR2-mediated high-speed mapping promises to revolutionize the understanding of brain circuitry by mapping of synaptic circuits in brain slices from transgenic mice expressing channelrhodopsin-2.
Optogenetic approaches for functional mouse brain mapping
- BiologyFront. Neurosci.
- 2013
Recently developed functional mapping methods that use optogenetic single-point stimulation in the rodent brain and employ cellular electrophysiology, evoked motor movements, voltage sensitive dyes (VSDs), calcium indicators, or functional magnetic resonance imaging (fMRI) to assess activity are reviewed.
Recent Developments in Optical Neuromodulation Technologies
- Biology, PsychologyMolecular Neurobiology
- 2012
An overview of the recent developments in the field of optogenetics technology that are relevant for a better understanding of several neuropsychiatric and neurodegenerative disorders and may pave the way for future therapeutic interventions is provided.
Laser-scanning photostimulation of optogenetically targeted forebrain circuits.
- BiologyJournal of visualized experiments : JoVE
- 2013
A protocol for examining the functional organization of forebrain circuits in vitro using laser-scanning photostimulation of channelrhodopsin, expressed optogenetically via viral-mediated transfection, and exploiting the utility of cre-lox recombination in transgenic mice to target expression in specific neuronal cell types is described.
Implantable optoelectronic probes for in vivo optogenetics.
- BiologyJournal of neural engineering
- 2017
The progress made in the field of hybrid optoelectronic neural interfaces that combine optical stimulation with electrophysiological recordings is reported, and advantages and fundamental limitations of various different designs are summarized.
Next-generation transgenic mice for optogenetic analysis of neural circuits
- BiologyFront. Neural Circuits
- 2013
These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of channelrhodopsins, behind various neuron-specific promoters, and permit photoinhibition or photostimulation both in vitro and in vivo.
Visualization of Synaptic Inhibition with an Optogenetic Sensor Developed by Cell-Free Protein Engineering Automation
- BiologyThe Journal of Neuroscience
- 2013
We describe an engineered fluorescent optogenetic sensor, SuperClomeleon, that robustly detects inhibitory synaptic activity in single, cultured mouse neurons by reporting intracellular chloride…
Recent advances in patterned photostimulation for optogenetics
- Physics
- 2017
An important technological revolution is underway in the field of neuroscience as we begin the 21st century. The combination of optical methods with genetically encoded photosensitive tools…
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