Options and considerations when selecting a quantitative proteomics strategy

  title={Options and considerations when selecting a quantitative proteomics strategy},
  author={B. Domon and R. Aebersold},
  journal={Nature Biotechnology},
  • B. Domon, R. Aebersold
  • Published 2010
  • Computer Science, Medicine
  • Nature Biotechnology
  • The vast majority of proteomic studies to date have relied on mass spectrometric techniques to identify, and in some cases quantify, peptides that have been generated by proteolysis. Current approaches differ in the types of instrument used, their performance profiles, the manner in which they interface with biological research strategies, and their reliance on and use of prior information. Here, we consider the three main mass spectrometry (MS)-based proteomic approaches used today: shotgun… CONTINUE READING
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