Optimum reaction conditions for human lactate dehydrogenase isoenzymes as they affect total lactate dehydrogenase activity.

@article{Gay1968OptimumRC,
  title={Optimum reaction conditions for human lactate dehydrogenase isoenzymes as they affect total lactate dehydrogenase activity.},
  author={R. Gay and R. Mccomb and G. Bowers},
  journal={Clinical chemistry},
  year={1968},
  volume={14 8},
  pages={
          740-53
        }
}
Optimum reaction conditions at 30° ± 0.5° for two continuous spectrophotometric assay procedures, lactate to pyruvate (L → P) and pyruvate to lactate (P → L), were determined with respect to pH at 30° (pH 30 ) substrate concentration, and coenzyme concentration for the human LDH isoenzymes. For the P → L procedure, broad pH 30 optima were within the range of 7.20-7.40 for all the LDH isoenzymes. The coenzyme optima were identical for all of the isoenzymes tested at a reduced NAD concentration… Expand
209 Citations
Table 1 . Assay Conditions for Lactate Dehydrogenase
  • PDF
Measurement of total lactate dehydrogenase activity.
  • R. Vanderlinde
  • Biology, Medicine
  • Annals of clinical and laboratory science
  • 1985
  • 76
UV-Assay with Pyruvate and NADH
  • 554
Lactate Dehydrogenase and its Isoenzymes
  • 1
Biochemical and Enzymological Study of Lactate Dehydrogenase Isoenzymes from Commercial Quality Control Sera and Several Animal Tissue Sources
  • 8
  • PDF
Lactate Dehydrogenase Inhibitorsin NADH Preparations
  • PDF
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 31 REFERENCES
pH Dependence of Lactate Dehydrogenase Isozyme Inhibition by Substrate
  • 30
Localization of Lactic Acid Dehydrogenase Activity in Serum Fractions
  • E. Vesell, A. Bearn
  • Chemistry, Medicine
  • Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1957
  • 170
SERUM LACTIC DEHYDROGENASE ACTIVITY: AN ANALYTICAL ASSESSMENT OF CURRENT ASSAYS.
  • 369
  • PDF
A comparison of reduced-NAD preparations from four commercial sources.
  • 20
  • PDF
The staining of lactic dehydrogenase isoenzymes after electrophoretic separation on cellulose acetate
  • H. Barnett
  • Medicine
  • Journal of clinical pathology
  • 1964
  • 50
  • PDF
An introduction to diagnostic enzymology.
  • 35
Elevation of a serum component in neoplastic disease.
  • 202
  • PDF
SERUM ENZYMES IN THE PHYSIOPATHOLOGY OF MUSCLE
  • 88
Rapid cellulose acetate electrophoresis. I. Serum proteins.
  • 22
  • PDF
...
1
2
3
4
...