Optimum Conditions Of 5-Nucleotidase Activity In Sera Of KalaAzaric Patients

  • Eaman A.S. AL-Rubaee
  • Published 2012

Abstract

Background: This research focuses on the characterization studies of 5-Nucleotidase (5'-NT) of kalaazar patients' sera. 5V NT is one of the enzymes used as indicator of liver function test . In this study the optimum conditions for the activity of 5s NT enzyme has been demonstrated for kalaazaric patients. Materials and methods: Thirty five blood samples were obtained from Baghdad Hospitals from untreated infants and children with kala-azar. 5'-NT activity was measured by following the method of Wood and Williams. Results: Optimum conditions of serum 5' NT activity of kalaazaric patients obtained were 50 u L , 30 minutes ,0.8 mM,7.7 and 50'C) as (Volume of serum , incubation time , substrate concentration, pH and temperature) respectively

Cite this paper

@inproceedings{ALRubaee2012OptimumCO, title={Optimum Conditions Of 5-Nucleotidase Activity In Sera Of KalaAzaric Patients}, author={Eaman A.S. AL-Rubaee}, year={2012} }