Optimizing the purification and analysis of miRNAs from urinary exosomes.

  title={Optimizing the purification and analysis of miRNAs from urinary exosomes.},
  author={Sarath Kiran Channavajjhala and Marzia Rossato and Francesca Morandini and Annalisa Castagna and Francesca Pizzolo and Flavia Bazzoni and Oliviero Olivieri},
  journal={Clinical chemistry and laboratory medicine},
  volume={52 3},
BACKGROUND Exosomes are cytoplasm containing vesicles released by many cells that can be found in several biological fluids including urine. Urinary exosomes are released from every segment of the nephron, are detectable in urine, constitutively contain RNA (small RNAs and mRNAs) and harbor unique subset of proteins, reflecting their cellular source. METHODS With the aim of establishing the optimal protocol for high throughput analysis of exosomal miRNAs, we compared three different urinary… CONTINUE READING
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