Optimizing the expression of recombinant alphabetagamma GABAA receptors in HEK293 cells for high-throughput screening.

@article{Gilbert2009OptimizingTE,
  title={Optimizing the expression of recombinant alphabetagamma GABAA receptors in HEK293 cells for high-throughput screening.},
  author={Daniel Frederic Gilbert and Abolghasem Esmaeili and Joseph W Lynch},
  journal={Journal of biomolecular screening},
  year={2009},
  volume={14 1},
  pages={86-91}
}
Despite being important clinical targets, it is not straightforward to reliably express recombinant trimeric alphabetagamma GABA-A receptors (GABA(A)Rs) for high-throughput screening. This study therefore sought to devise a simple and reliable means of transiently expressing alpha1beta1gamma1 and alpha1beta1gamma2 GABA(A)Rs in HEK293 cells. Expression efficiencies resulting from 5 different transfection strategies were assessed by flow cytometry and pharmacological analysis using an anion… CONTINUE READING

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