Optimizing separation conditions for riboflavin, flavin mononucleotide and flavin adenine dinucleotide in capillary zone electrophoresis with laser-induced fluorescence detection.

@article{Catald2002OptimizingSC,
  title={Optimizing separation conditions for riboflavin, flavin mononucleotide and flavin adenine dinucleotide in capillary zone electrophoresis with laser-induced fluorescence detection.},
  author={Tommaso R I Catald and Donatella Nardiello and Giuseppe Egidio De Benedetto and Sabino A. Bufo},
  journal={Journal of chromatography. A},
  year={2002},
  volume={968 1-2},
  pages={229-39}
}
A method was developed for the quantitative determination of riboflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), using free solution capillary zone electrophoresis in uncoated fused-silica capillaries with laser-induced fluorescence (LIF) detection. Various factors influencing the separation and detection of flavin vitamers were investigated, including pH (5.5-10.5), concentration and nature of the run buffer (phosphate, borate and carbonate), applied voltage (15-30… CONTINUE READING