Optimizing pressurized liquid extraction of microbial lipids using the response surface method.

@article{Cescut2011OptimizingPL,
  title={Optimizing pressurized liquid extraction of microbial lipids using the response surface method.},
  author={Julien Cescut and Etienne S{\'e}verac and Carole Molina-Jouve and J-L Uribelarrea},
  journal={Journal of chromatography. A},
  year={2011},
  volume={1218 3},
  pages={373-9}
}
Response surface methodology (RSM) was used for the determination of optimum extraction parameters to reach maximum lipid extraction yield with yeast. Total lipids were extracted from oleaginous yeast (Rhodotorula glutinis) using pressurized liquid extraction (PLE). The effects of extraction parameters on lipid extraction yield were studied by employing a second-order central composite design. The optimal condition was obtained as three cycles of 15 min at 100°C with a ratio of 144 g of… CONTINUE READING