Optimizing plasmid-based gene transfer for investigating skeletal muscle structure and function.

@article{Schertzer2006OptimizingPG,
  title={Optimizing plasmid-based gene transfer for investigating skeletal muscle structure and function.},
  author={Jonathan D Schertzer and David R. Plant and Gordon S Lynch},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2006},
  volume={13 4},
  pages={795-803}
}
Intramuscular injection of naked plasmid DNA is a less cytotoxic alternative to viral vectors for delivering genetic material to skeletal muscle in vivo. However, the low efficiency of plasmid-based gene transfer limits its potential therapeutic efficacy and/or its use for many experimental applications. Current strategies to enhance transfection efficiency (i.e., electroporation) can cause significant muscle damage, confounding physiological assessments such as muscle contractility. Optimizing… CONTINUE READING

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