Optimizing photoheterotrophic H2 production by Rhodobacter capsulatus upon interposon mutagenesis in the hupL gene

@article{Jahn1994OptimizingPH,
  title={Optimizing photoheterotrophic H2 production by Rhodobacter capsulatus upon interposon mutagenesis in the hupL gene},
  author={Andreas Jahn and Barbara Keuntje and Michael D{\"o}rffler and Werner Klipp and Juergen Oelze},
  journal={Applied Microbiology and Biotechnology},
  year={1994},
  volume={40},
  pages={687-690}
}
In Rhodobacter capsulatus, the hupL gene encoding the large subunit of the uptake-hydrogenase (Hup) enzyme complex was mutated by insertion of an interposon. The mutant neither synthesized an active hydrogenase nor grew photoautotrophically. Under conditions of nitrogen (N) limitation, photoheterotrophic cultures of the wild type and the mutant evolved H2 by activity of the nitrogenase enzyme complex. When grown with glutamate as an N source and either d,l-malate or l-lactate as carbon sources… CONTINUE READING
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