Optimizing environmental factors for large-scale multiplication of chrysanthemum (Chrysanthemum grandiflorum) in balloon-type bioreactor culture

@article{Sivakumar2007OptimizingEF,
  title={Optimizing environmental factors for large-scale multiplication of chrysanthemum (Chrysanthemum grandiflorum) in balloon-type bioreactor culture},
  author={Ganapathy Sivakumar and Sun Ja Kim and Eun Joo Hahn and Kee Yoeup Paek},
  journal={In Vitro Cellular & Developmental Biology - Plant},
  year={2007},
  volume={41},
  pages={822-825}
}
We have developed optimum culture conditions for the large-scale propagation of chrysanthemum in balloon-type bioreactors to achieve vigorous growth and quality. The effects of NH 4 + /NO 3 − ratio, air volume, air temperature, photosynthetic photo flux, and an inoculation density on the growth and quality of plantlets were investigated. The best production conditions were an NH 4 + :NO 3 − ratio of 20∶40 mM, air exchange of 0.1 vvm min−1, air temperature 25°C, photosynthetic photo flux (PPF… CONTINUE READING