Optimizing electroporation conditions in primary and other difficult-to-transfect cells.

  title={Optimizing electroporation conditions in primary and other difficult-to-transfect cells.},
  author={Elizabeth T Jordan and Michelle E Collins and Joseph Terefe and Luis A. Ugozzoli and Teresa Rodr{\'i}guez Rubio},
  journal={Journal of biomolecular techniques : JBT},
  volume={19 5},
Electroporation is a valuable tool for nucleic acid delivery because it can be used for a wide variety of cell types. Many scientists are shifting toward the use of cell types that are more relevant to in vivo applications, including primary cells, which are considered difficult to transfect. The ability to electroporate these cell types with nucleic acid molecules of interest at a relatively high efficiency while maintaining cell viability is essential for elucidating the pathway(s) in which a… CONTINUE READING
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