Corpus ID: 88187527

Optimizing conditions for PCR amplification of DNA with rich GC

@inproceedings{Bu2002OptimizingCF,
  title={Optimizing conditions for PCR amplification of DNA with rich GC},
  author={Zhang Bu},
  year={2002}
}
  • Zhang Bu
  • Published 2002
  • Biology
  • Objective:To determine the optimized conditions for PCR amplification of DNA with rich GC in order to freely assemble modules or domains of polyketide synthase (PKS) in macrolide combinatorial biosynthesis.Methods:Formamide, glycerol, DMSO and Mg 2+ were added to the PCR amplification system and amplification system was chosen to optimize the conditions for PCR amplification of DNA with rich GC.Results:Formamide had no influence on PCR amplification of DNA with rich GC, while both glycerol and… CONTINUE READING

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