Optimizing bioconversion pathways through systems analysis and metabolic engineering.

@article{Stafford2002OptimizingBP,
  title={Optimizing bioconversion pathways through systems analysis and metabolic engineering.},
  author={Daniel E Stafford and Kurt S Yanagimachi and Philip A. Lessard and Sushil K Rijhwani and Anthony J Sinskey and Gregory Stephanopoulos},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99 4},
  pages={1801-6}
}
We demonstrate a general approach for metabolic engineering of biocatalytic systems comprising the uses of a chemostat for strain improvement and radioisotopic tracers for the quantification of pathway fluxes. Flux determination allows the identification of target pathways for modification as validated by subsequent overexpression of the corresponding gene. We demonstrate this method in the indene bioconversion network of Rhodococcus modified for the overproduction of 1,2-indandiol, a key… CONTINUE READING
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Molecular Cloning: A Laboratory Manual (Cold

  • J. Sambrook, E. F. Fritsch, T. Maniatis
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