Optimized single-step affinity purification with a self-cleaving intein applied to human acidic fibroblast growth factor.

@article{Wood2000OptimizedSA,
  title={Optimized single-step affinity purification with a self-cleaving intein applied to human acidic fibroblast growth factor.},
  author={David P. Wood and Victoria Derbyshire and W Wu and Michel Chartrain and Marlene Belfort and Georges Belfort},
  journal={Biotechnology progress},
  year={2000},
  volume={16 6},
  pages={1055-63}
}
To reduce the number of recovery steps during downstream processing and to overcome the limitations of present fusion-based affinity separations, a controllable self-splicing protein element in the form of a mini-intein was used to optimize the recovery of proteins for both batch and flow purification strategies. The ability to recover purified proteins was demonstrated using a tripartite fusion consisting of a maltose binding domain, a truncated intein as a controllable linker molecule, and a… CONTINUE READING