Optimized signal peptides for the development of high expressing CHO cell lines

@article{Kober2013OptimizedSP,
  title={Optimized signal peptides for the development of high expressing CHO cell lines},
  author={Lars Kober and Christoph Zehe and Juergen Bode},
  journal={Biotechnology and Bioengineering},
  year={2013},
  volume={110}
}
Recombinant biotherapeutic proteins such as monoclonal antibodies are mostly produced in Chinese hamster ovary (CHO) cells and pharmaceutical companies are interested in an appropriate platform technology for the development of large‐scale production processes. A major aim of our study was therefore to improve the secretion efficiency of a recombinant biotherapeutic antibody by optimizing signal peptides. Reporter molecules such as gaussia and vargula luciferase or secreted alkaline phosphatase… 

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