Optimized sample preparation for single-molecule localization-based superresolution microscopy in yeast

@article{Kaplan2015OptimizedSP,
  title={Optimized sample preparation for single-molecule localization-based superresolution microscopy in yeast},
  author={Charlotte Kaplan and Helge Ewers},
  journal={Nature Protocols},
  year={2015},
  volume={10},
  pages={1007-1021}
}
Single-molecule localization-based superresolution microscopy methods allow the resolution of cellular structures in the range of tens of nanometers. However, these techniques are of limited use in current yeast labeling protocols, owing to problems with structural preservation. Here we describe an optimized sample preparation protocol that enables single-molecule localization microscopy at high resolution combined with improved structural preservation in Saccharomyces cerevisiae. The protocol… CONTINUE READING
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