Optimized sample preparation for MALDI mass spectrometry analysis of protected synthetic peptides

@article{Schaiberger2008OptimizedSP,
  title={Optimized sample preparation for MALDI mass spectrometry analysis of protected synthetic peptides},
  author={Audrey M Schaiberger and J. Moss},
  journal={Journal of the American Society for Mass Spectrometry},
  year={2008},
  volume={19},
  pages={614-619}
}
The recent development and commercialization of Fuzeon (enfuvirtide) demonstrated that a convergent strategy comprised of both solid- and solution-phase synthetic methodologies presents a viable route for peptide manufacturing on a multi-ton scale. In this strategy, the target sequence is prepared by stepwise solid-phase synthesis of protected peptide fragments, which are then coupled together in the solution-phase to give the full-length sequence. These synthetic methodologies pose a unique… Expand
Development of novel mass spectrometry-based approaches for searching for low-mass tyrosinase inhibitors in complex mixtures
This thesis report presents the development of mass spectrometry-based methods for searching for inhibitors of enzymes in complex mixtures, such as plant extracts. Tyrosinase enzyme was used as theExpand
Characterization of Synthetic Peptides by Mass Spectrometry.
TLDR
Simple procedures are described for MALDI-TOF-MS and LC-MS of synthetic peptides to confirm the identity and purity of the peptide. Expand
Quantification of the PR-39 cathelicidin compound in porcine blood by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
TLDR
The MALDI method was successfully applied for the quantitative analysis of PR-39 found in porcine blood, and has lower accuracy than the enzyme-linked immunosorbent assay (ELISA). Expand
Taking advantage of oxidation to characterize thiol-containing polymer chains by MALDI-TOF mass spectrometry.
TLDR
Performing MALDI-TOF mass spectrometry analyses in oxidizing conditions, as complement to regular analyses, was found to be very useful for the chain-end identification of different thiol-containing polymer chains. Expand
A useful binary matrix for visible-MALDI of low molecular weight analytes
In this work, a new absorbing candidate, rhodamine (R) 575, is described, which forms the basis of a binary matrix operating at 532 nm. Analyte ionization is found to be much more efficient when theExpand
Tunable fragmentation of organic molecules in laser ablation glow discharge time-of-flight mass spectrometry
TLDR
It was found that LAGD offers tunable ionization and provides a reduced matrix dependence, however, the sensitivity achieved by the prototype LAGd-TOFMS was significantly lower when compared with commercially available MALDI-toFMS instrumentation. Expand
Polymer Molecular Weight Analysis by 1H NMR Spectroscopy
The measurement and analysis of molecular weight and molecular weight distribution remain matters of fundamental importance for the characterization and physical properties of polymers. GelExpand
Compatibility of Maximum-Containment Virus-Inactivation Protocols With Identification of Bacterial Coinfections by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.
TLDR
A panel of bacteria with 6 validated maximum-containment virus-inactivation protocols is assessed and it is reported that inactivation with TRIzol or γ-irradiation is compatible with MALDI-TOF MS. Expand
Synthetic Progress in cMyc‐Max Oncoprotein Miniaturization: Semi‐Online Monitoring Gives Solid‐Phase Access to Hydrophobic b(‐HLH‐)ZIP Peptidosteroid Tweezers
Miniature versions of basic leucine zipper (bZIP) and basic helix-loop-helix zipper (b-HLH-ZIP) transcription factors are promising tools for molecular dissection of the genetic information in aExpand
Chiral dendrigraft polymer for asymmetric synthesis of isoquinuclidines
The synthesis of multi-arm star dendrigraft amidoamine polymer with pentaerythritol initiated polyepichlorohydrin as the core, PEN-G2, on a solid resin support has been demonstrated. We haveExpand
...
1
2
...

References

SHOWING 1-10 OF 25 REFERENCES
Selective sampling of phosphopeptides for detection by MALDI mass spectrometry.
TLDR
A simple nonsorptive method to isolate phosphopeptides based on their pI and knowledge of pI based on the rejection/injection of peptides was found to be useful in peak assignment. Expand
Evaluation of matrix-assisted laser desorption ionization mass spectrometry for polymer characterization
TLDR
A protocol for the preparation of polymeric samples for time-of-flight matrix-assisted laser desorption ionization mass spectrometry (TOF-MALDI-MS) analysis was developed, and the addition of silver for cationization of molecules was determined to be necessary. Expand
Basic matrices for the matrix-assisted laser desorption/ionization mass spectrometry of proteins and oligonucleotides.
TLDR
37 highly substituted pyrimidine, pyridine, and benzene derivatives containing basic amino groups were screened as potential matrices and found to be useful for the preparation and analysis of samples under neutral and basic pH conditions. Expand
On-probe sample preparation without washes for matrix-assisted laser desorption/ionization mass spectrometry using an anion exchange medium.
TLDR
An on-probe sample preparation method without washes for MALDI, using a strong anion exchange silica gel, which is applicable even in the presence of 2.5% SDS, and is not only very simple but also inexpensive, because it can be used with the standard MAL DI target plates. Expand
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS).
  • J. Stults
  • Chemistry, Medicine
  • Current opinion in structural biology
  • 1995
TLDR
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been responsible for solving many problems in structural biology and is poised to improve in performance and in its usefulness for current and new applications. Expand
Analysis of hydrocarbon polymers by matrix-assisted laser desorption / ionization-fourier transform mass spectrometry
A quick and effective sample preparation is demonstrated for matrix-assisted laser desorption/ionization (MALDI) analysis of nonpolar polymers. Polyisoprene, polystyrene, and polybutadiene polymersExpand
2,5-Dihydroxybenzoic acid: a new matrix for laser desorption—ionization mass spectrometry
The performance of 2,5-dihydroxybenzoic acid (DHB) as a matrix for laser desorption—ionization mass spectrometry of proteins is described. Samples from protein/matrix mixtures with mass ratiosExpand
Signal amplification using "spot-on-a-chip" technology for the identification of proteins via MALDI-TOF MS.
The presented "spot-on-a-chip" technology enables easy enrichment of samples in the low nanomolar (1-5 nM) range and provides a fast and reliable automated sample preparation method for performingExpand
Mass spectrometry of intracellular and membrane proteins using cleavable detergents.
TLDR
A novel cleavable detergent, PPS, was found to improve sensitivity in MALDI analyses of both soluble proteins and membrane proteins without degrading spectral quality and was applied to whole cell extracts. Expand
A MALDI sample preparation method suitable for insoluble polymers
TLDR
This sample preparation is compared with the common dried droplet sample preparation method and found to perform much better, both in terms of robustness against variation of experimental parameters and high-mass capability. Expand
...
1
2
3
...