Optimized protocols for the detection of porcine circovirus 2 DNA from formalin-fixed paraffin-embedded tissues using nested polymerase chain reaction and comparison of nested PCR with in situ hybridization.

@article{Kim2001OptimizedPF,
  title={Optimized protocols for the detection of porcine circovirus 2 DNA from formalin-fixed paraffin-embedded tissues using nested polymerase chain reaction and comparison of nested PCR with in situ hybridization.},
  author={J H Kim and Chanhee Chae},
  journal={Journal of virological methods},
  year={2001},
  volume={92 2},
  pages={105-11}
}
Optimized DNA extraction method and nested polymerase chain reaction (PCR) were developed for the detection of porcine circovirus 2 (PCV2) from formalin-fixed, paraffin-embedded tissues. Conventional PCR, nested PCR, and in situ hybridization methods were also compared for the detection of PCV2 in archival tissues. A method based on xylene deparaffinization… CONTINUE READING