Optimized nitrate reductase assay predicts the rate of nitrate utilization in the halotolerant microalga Dunaliella viridis

@article{Javier2004OptimizedNR,
  title={Optimized nitrate reductase assay predicts the rate of nitrate utilization in the halotolerant microalga Dunaliella viridis},
  author={Francisco Javier and Luz A. Gordillo and Carlos Jimenez and Alfonso Corzo and Francisco Xavier Niell},
  journal={Journal of Applied Phycology},
  year={2004},
  volume={9},
  pages={99-106}
}
An in situ method for measuring nitrate reductase (NR) activity in Dunaliella viridis was optimized in terms of incubation time, concentration of KNO3, permeabilisers (1-propanol and toluene), pH, salinity, and reducing power (glucose and NADH). NR activity was measured by following nitrite production and was best assayed with 50 mM KNO3, 1.2 mM NADH, 5% 1-propanol (v/v), at pH 8.5. The estimated half-saturation constant (Ks) for KNO3 was 5 mM. Glucose had no effect as external reducing power… CONTINUE READING