Optimized microplate beta-1,3-glucanase assay system for Trichoderma spp. screening.

  title={Optimized microplate beta-1,3-glucanase assay system for Trichoderma spp. screening.},
  author={Marcelo Henrique Soller Ramada and Fabyano Alvares Cardoso Lopes and Cirano Jos{\'e} Ulhoa and Roberto Nascimento Silva},
  journal={Journal of microbiological methods},
  volume={81 1},
Beta-1,3-glucanase is an important cell wall-degrading enzyme involved in mycoparasitism by Trichoderma spp. during antagonism against phytopathogenic fungi. A simple microplate-based method to assay beta-1,3-glucanase activity is described here as an alternative to an expensive tube-assay method. The reaction volume of the micro-assay was reduced to 130 microl from the 1150 microl used in the standard beta-1,3-glucanase macro-assay. Statistical analyses showed significant difference in… CONTINUE READING

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