Optimized green fluorescent protein fused to FoF1-ATP synthase for single-molecule FRET using a fast anti-Brownian electrokinetic trap

@inproceedings{Dienerowitz2016OptimizedGF,
  title={Optimized green fluorescent protein fused to FoF1-ATP synthase for single-molecule FRET using a fast anti-Brownian electrokinetic trap},
  author={M. Dienerowitz and M. Ilchenko and B. Su and G. Deckers-Hebestreit and G. Mayer and T. Henkel and T. Heitkamp and M. B{\"o}rsch},
  booktitle={SPIE BiOS},
  year={2016}
}
  • M. Dienerowitz, M. Ilchenko, +5 authors M. Börsch
  • Published in SPIE BiOS 2016
  • Materials Science, Engineering, Biology, Chemistry
  • Observation times of freely diffusing single molecules in solution are limited by the photophysics of the attached fluorescence markers and by a small observation volume in the femtolitre range that is required for a sufficient signal-to-background ratio. To extend diffusion-limited observation times through a confocal detection volume, A. E. Cohen and W. E. Moerner have invented and built the ABELtrap — a microfluidic device to actively counteract Brownian motion of single nanoparticles with… CONTINUE READING
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