Optimized expression of the Starmerella bombicola lactone esterase in Pichia pastoris through temperature adaptation, codon-optimization and co-expression with HAC1.

@article{Waele2018OptimizedEO,
  title={Optimized expression of the Starmerella bombicola lactone esterase in Pichia pastoris through temperature adaptation, codon-optimization and co-expression with HAC1.},
  author={Stijn De Waele and Isabel Vandenberghe and Bram Laukens and S{\"o}ren Planckaert and Stijn Verweire and Inge N A Van Bogaert and Wim K. Soetaert and Bart Devreese and Katarzyna Ciesielska},
  journal={Protein expression and purification},
  year={2018},
  volume={143},
  pages={62-70}
}
The Starmerella bombicola lactone esterase (SBLE) is a novel enzyme that, in vivo, catalyzes the intramolecular esterification (lactonization) of acidic sophorolipids in an aqueous environment. In fact, this is an unusual reaction given the unfavorable conditions for dehydration. This characteristic strongly contributes to the potential of SBLE to become a… CONTINUE READING