Optimized experimental design for the estimation of enzyme kinetic parameters: an experimental evaluation.

@article{Sjgren2012OptimizedED,
  title={Optimized experimental design for the estimation of enzyme kinetic parameters: an experimental evaluation.},
  author={Erik Sj{\"o}gren and Petter Svanberg and Kajsa P Kanebratt},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2012},
  volume={40 12},
  pages={2273-9}
}
A set of compounds (n = 30), including traditional cytochrome P450 substrates and compounds from AstraZeneca's compound library, was used in an experimental evaluation of an optimal design approach (ODA) for the estimation of enzyme kinetic parameters (CL(int), V(max), and K(m)). A depletion method previously shown to provide reliable results, the multiple depletion curves method (MDCM), was used as reference. Experiments were conducted with human liver microsomes, and samples were analyzed… CONTINUE READING

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